This research set out to determine whether or not the crude methanolic extract of A. santolinifolia Turcz. Ex Besser possessed any phytochemical, antioxidant, or antibacterial properties. The antioxidant activity was measured with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzthiazoline-6- sulphonic acid (ABTS) assays, with the methanolic extract of Artemisia santolinifolia root showing the highest scavenging activity (DPPH) at 61.31 g/ml and the leaves showing the lowest at 51. Similarly, A. santolinifolia roots showed the highest activity in (ABTS) (89.16 g/ml), whereas leaves showed the lowest activity (68.14 g/ml). At doses as low as 25 g/ml, 50 g/ml, and 100 g/ml, crude methanolic extracts demonstrated substantial suppression of all tested microorganisms. A. santolinifolia AsL leaf extract demonstrated MIC values of 12.5 g/ml against gram-positive bacteria B. subtilis, 50 g/ml against gram-positive bacteria S. aureus, and 37.5 g/ml against gram-negative bacteria P. aeruginosa, which are all very close to the response of conventional ciprofloxacin. Based on the results of the current study, we recommend using Artemisia santolinifolia root (AsR) for the treatment and management of various infectious diseases because of its high antioxidant capacity and Artemisia santolinifolia leaf (AsL) because of its good antibacterial activity.

Antioxidant Potential, Antibacterial Activity, Phytochemicals, Artemisia santolinifolia.