Antibiotic-resistant bacteria are difficult to cure, but there may be new hope in the form of medicines that can be extracted from medicinal plants. The researchers set out to determine if essential oil from Pimpinella anisum could inhibit the growth of Pseudomonas aeruginosa and Bacillus subtilis, two common types of bacteria. Chemical composition was determined using gas chromatography mass spectrometry. Agar disk and agar well diffusion methods were used as a preliminary screen for the essential oil's antibacterial capabilities. To find the MIC, a Macrobroth tube test was used. The results showed that Trans-anethole made up the majority of the substances in P. anisum essential oil (89.7%), and that P. anisum essential oil at doses of 0.003 and 0.007 g/ml inhibited the growth of P. aeruginosa and B. subtilis, respectively. The findings demonstrate, then, that the ethnomedical plant has antibacterial properties against the two microorganisms studied. The findings support the idea that the plant's essential oil has therapeutic and/or preservative potential. Further in vivo research and clinical trials are required for justification. The possibility of it being used as an antibacterial agent in topical or oral treatments also needs more study. Future research will focus on the fractionation and characterisation of active compounds.

Pimpinella anisum, Essential oil, Chemical composition, Antibacterial effect.